Hotspot

psbB

LSC · 72596-74122 · 1527 bp

Back to Scolopia

Pi 0.00196

MarkerSeek score 59.80

Primer available yes

Label psbB

Feature Metadata

label_name: psbB
feature_type: gene
region: LSC
length_bp: 1527
variable_sites: 3
pi: 0.00196
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB_p1	ATGGAATCCTTTCTCCGAGA	GTCCCCAGTACCCTATGTCA	45.0	55.0	55.6	57.8	1707 bp	1.0	59.8
psbB_p2	ATGGAATCCTTTCTCCGAGA	TGTCCCCAGTACCCTATGTC	45.0	55.0	55.6	57.8	1708 bp	1.0	59.8
psbB_p3	TGGAATCCTTTCTCCGAGAT	GTCCCCAGTACCCTATGTCA	45.0	55.0	55.6	57.8	1706 bp	1.0	59.8
psbB_p4	TGGAATCCTTTCTCCGAGAT	TGTCCCCAGTACCCTATGTC	45.0	55.0	55.6	57.8	1707 bp	1.0	59.8
psbB_p5	TGGAATCCTTTCTCCGAGAT	AAAAGGCGGCGATTCAAATC	45.0	45.0	55.6	57.7	1731 bp	1.0	58.9