Hotspot

clpP

LSC · 70062-72115 · 2054 bp

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Pi 0.00195

MarkerSeek score 58.80

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2054
variable_sites: 4
pi: 0.00195
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	TCGCGATTTGTGTGAAATTCC	ACGCTTTCACATCAAAGTGA	42.9	40.0	57.8	55.9	2135-2136 bp	1.0	53.9
clpP_p2	CGCGATTTGTGTGAAATTCCT	ACGCTTTCACATCAAAGTGA	42.9	40.0	57.8	55.9	2134-2135 bp	1.0	53.9
clpP_p3	CGCGATTTGTGTGAAATTCC	ACGCTTTCACATCAAAGTGA	45.0	40.0	56.3	55.9	2134-2135 bp	1.0	50.1
clpP_p4	CCTAAATCGCGATTTGTGTGA	ACGCTTTCACATCAAAGTGA	42.9	40.0	57.0	55.9	2141-2142 bp	1.0	47.4
clpP_p5	TCCTAAATCGCGATTTGTGTG	ACGCTTTCACATCAAAGTGA	42.9	40.0	57.0	55.9	2142-2143 bp	1.0	47.4