Hotspot

rpl22-rps19

IRb · 84331-84447 · 117 bp

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Pi 0.0427

MarkerSeek score 66.40

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: IRb
length_bp: 117
variable_sites: 5
pi: 0.0427
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	GCAGATGCTTCATATGGGTTC	AATTTCCGGGGACATGCAAA	47.6	45.0	57.1	58.4	203-242 bp	1.0	63.6
rpl22-rps19_p2	GCAGATGCTTCATATGGGTTC	ATTTCCGGGGACATGCAAAA	47.6	45.0	57.1	58.4	202-241 bp	1.0	63.6
rpl22-rps19_p3	GCAGATGCTTCATATGGGTTC	TCTCAATTTCCGGGGACATG	47.6	50.0	57.1	57.6	207-246 bp	1.0	63.1
rpl22-rps19_p4	GCAGATGCTTCATATGGGTTC	GTGGGCCATAAATTGGGAGA	47.6	50.0	57.1	57.5	239-278 bp	1.0	62.6
rpl22-rps19_p5	TATTGCCCTAAAGCAGATGC	ATTTCCGGGGACATGCAAAA	45.0	45.0	55.9	58.4	214-253 bp	1.0	61.8