Hotspot

petB-petD

LSC · 76538-77516 · 979 bp

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Pi 0.00104

MarkerSeek score 61.10

Primer available yes

Label petB-petD

Feature Metadata

label_name: petB-petD
feature_type: igs
region: LSC
length_bp: 979
variable_sites: 1
pi: 0.00104
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB-petD_p1	AAGCAAGGTATTTCGGGTCC	ACCCATTCCTTTGGCCAATT	50.0	45.0	57.9	58.0	1099-1117 bp	1.0	80.0
petB-petD_p2	GCAAGGTATTTCGGGTCCTT	ACCCATTCCTTTGGCCAATT	50.0	45.0	57.9	58.0	1097-1115 bp	1.0	80.0
petB-petD_p3	AAGCAAGGTATTTCGGGTCC	GACCCATTCCTTTGGCCAAT	50.0	50.0	57.9	58.4	1100-1118 bp	1.0	77.0
petB-petD_p4	GCAAGGTATTTCGGGTCCTT	GACCCATTCCTTTGGCCAAT	50.0	50.0	57.9	58.4	1098-1116 bp	1.0	77.0
petB-petD_p5	GCAAGGTATTTCGGGTCCTT	TATGACCCATTCCTTTGGCC	50.0	50.0	57.9	57.5	1101-1119 bp	1.0	76.8