Hotspot

accD-psaI

LSC · 58826-59485 · 660 bp

Back to Scolopia

Pi 0.00303

MarkerSeek score 60.50

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 660
variable_sites: 2
pi: 0.00303
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GAGTTATTTCAGCTCCACGC	CCATTGCAATTGCCGGAAAT	50.0	45.0	56.9	58.0	796 bp	1.0	71.2
accD-psaI_p2	GAGTTATTTCAGCTCCACGC	TTGCCGGAAATACTAAGCCC	50.0	50.0	56.9	58.0	787 bp	1.0	71.1
accD-psaI_p3	GAGTTATTTCAGCTCCACGC	AAGAAGCCATTGCAATTGCC	50.0	45.0	56.9	57.9	802 bp	1.0	70.6
accD-psaI_p4	TGAGTTATTTCAGCTCCACGC	CCATTGCAATTGCCGGAAAT	47.6	45.0	58.7	58.0	797 bp	1.0	66.8
accD-psaI_p5	TGAGTTATTTCAGCTCCACGC	TTGCCGGAAATACTAAGCCC	47.6	50.0	58.7	58.0	788 bp	1.0	66.6