Hotspot

rbcL-accD

LSC · 56587-57328 · 742 bp

Back to Scolopia

Pi 0.00404

MarkerSeek score 61.10

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 742
variable_sites: 3
pi: 0.00404
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GCAAATGGAGCCCTGAACTA	CGGGTCTTCACTTACACTGG	50.0	55.0	57.9	57.9	945 bp	1.0	78.6
rbcL-accD_p2	TAGCAAATGGAGCCCTGAAC	CGGGTCTTCACTTACACTGG	50.0	55.0	57.9	57.9	947 bp	1.0	78.6
rbcL-accD_p3	TTCGTGAGGCTAGCAAATGG	CGGGTCTTCACTTACACTGG	50.0	55.0	58.3	57.9	957 bp	1.0	77.5
rbcL-accD_p4	CTTGCTCGTGAGGGTAATGT	CGGGTCTTCACTTACACTGG	50.0	55.0	57.6	57.9	982 bp	1.0	76.5
rbcL-accD_p5	AATGGAGCCCTGAACTAGCT	CGGGTCTTCACTTACACTGG	50.0	55.0	58.4	57.9	942 bp	1.0	76.3