Hotspot

petN-psbM

LSC · 27801-29104 · 1304 bp

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Pi 0.00234

MarkerSeek score 61.60

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1304
variable_sites: 3
pi: 0.00234
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.2	57.6	1446-1466 bp	1.0	68.8
petN-psbM_p2	CTTGGGCTGCTTTGATGGTA	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.2	57.6	1434-1454 bp	1.0	68.7
petN-psbM_p3	TTGGGCTGCTTTGATGGTAG	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.2	57.6	1433-1453 bp	1.0	68.7
petN-psbM_p4	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.6	57.6	1385-1405 bp	1.0	67.3
petN-psbM_p5	GGGGCCATAATTCACATGGA	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.6	57.6	1468-1488 bp	1.0	66.9