Hotspot

atpH-atpI

LSC · 12684-13698 · 1015 bp

Back to Scolopia

Pi 0.00394

MarkerSeek score 47.50

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1015
variable_sites: 4
pi: 0.00394
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	CTCGCGATACCTTCTACAGC	TATGGTCCATGGGTTCCCTT	55.0	50.0	58.0	58.0	1611 bp	1.0	79.9
atpH-atpI_p2	CTCGCGATACCTTCTACAGC	GTGAATCCATGGAGGGACAC	55.0	55.0	58.0	58.0	1163 bp	1.0	79.7
atpH-atpI_p3	CCCGTTGGTTTATTGTCCCA	TATGGTCCATGGGTTCCCTT	50.0	50.0	58.1	58.0	1885-1889 bp	1.0	79.6
atpH-atpI_p4	TCTCGTGTCCATTGCGAAAT	TATGGTCCATGGGTTCCCTT	45.0	50.0	57.9	58.0	2019-2023 bp	1.0	79.6
atpH-atpI_p5	CTCGCGATACCTTCTACAGC	ACCATTCCTACTGACGGTCA	55.0	50.0	58.0	58.1	1695 bp	1.0	79.4