Hotspot

trnQ-UUG-psbK

LSC · 6041-6395 · 355 bp

Back to Scolopia

Pi 0.00563
MarkerSeek score 42.90
Primer available yes
Label trnQ-UUG-psbK

Feature Metadata

label_name
trnQ-UUG-psbK
feature_type
igs
region
LSC
length_bp
355
variable_sites
2
pi
0.00563
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
42.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnQ-UUG-psbK_p1 CTTGGAGGTTCCCCTTCTCT CTTACAGCAGCTTGCCAAAC 55.0 50.0 58.3 57.9 1180 bp 0.5 56.9
trnQ-UUG-psbK_p2 CTTGGAGGTTCCCCTTCTCT AGGATTACGCCCTGGATCAT 55.0 50.0 58.3 58.3 1702 bp 0.5 55.2
trnQ-UUG-psbK_p3 CTTGGAGGTTCCCCTTCTCT AAACTTACAGCAGCTTGCCA 55.0 45.0 58.3 58.3 1183 bp 0.5 54.8
trnQ-UUG-psbK_p4 CTTGGAGGTTCCCCTTCTCT AACTTACAGCAGCTTGCCAA 55.0 45.0 58.3 58.3 1182 bp 0.5 54.8
trnQ-UUG-psbK_p5 CTTGGAGGTTCCCCTTCTCT ACTTACAGCAGCTTGCCAAA 55.0 45.0 58.3 58.3 1181 bp 0.5 54.8