Hotspot

matK-trnK-UUU

LSC · 3519-4274 · 756 bp

Back to Scolopia

Pi 0.00529
MarkerSeek score 61.80
Primer available yes
Label matK-trnK-UUU

Feature Metadata

label_name
matK-trnK-UUU
feature_type
igs
region
LSC
length_bp
756
variable_sites
4
pi
0.00529
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK-trnK-UUU_p1 ACTCCCGAAAGATAAGTGGG TGGGGTTGCTAACTCAATGG 50.0 50.0 56.6 57.8 880 bp 1.0 67.7
matK-trnK-UUU_p2 CTCCCGAAAGATAAGTGGGT TGGGGTTGCTAACTCAATGG 50.0 50.0 56.6 57.8 879 bp 1.0 67.7
matK-trnK-UUU_p3 ACTCCCGAAAGATAAGTGGG GGGGTTGCTAACTCAATGGT 50.0 50.0 56.6 57.8 879 bp 1.0 67.6
matK-trnK-UUU_p4 CTCCCGAAAGATAAGTGGGT GGGGTTGCTAACTCAATGGT 50.0 50.0 56.6 57.8 878 bp 1.0 67.6
matK-trnK-UUU_p5 ACTCCCGAAAGATAAGTGGGT TGGGGTTGCTAACTCAATGG 47.6 50.0 58.5 57.8 880 bp 1.0 67.0