Hotspot

rpl32-trnL-UAG

SSC · 113299-114002 · 704 bp

Back to Scleromitrion

Pi 0.0345
MarkerSeek score 71.90
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
704
variable_sites
24
pi
0.0345
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
71.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGACAGCGTTAAAAGCGTTT TCCTAAGAGCAGCGTGTCTA 45.0 50.0 57.6 57.9 824-832 bp 1.0 78.9
rpl32-trnL-UAG_p2 GGACAGCGTTAAAAGCGTTT AGCAGCGTGTCTACCAATTT 45.0 45.0 57.6 57.8 817-825 bp 1.0 78.5
rpl32-trnL-UAG_p3 GGACAGCGTTAAAAGCGTTT CCTAAGAGCAGCGTGTCTAC 45.0 55.0 57.6 57.8 823-831 bp 1.0 78.4
rpl32-trnL-UAG_p4 GGACAGCGTTAAAAGCGTTT CTAAGAGCAGCGTGTCTACC 45.0 55.0 57.6 57.8 822-830 bp 1.0 78.4
rpl32-trnL-UAG_p5 GGACAGCGTTAAAAGCGTTT GAGCAGCGTGTCTACCAATT 45.0 50.0 57.6 58.3 818-826 bp 1.0 77.8