Hotspot

ndhF-rpl32

SSC · 112302-113133 · 832 bp

Back to Scleromitrion

Pi 0.035

MarkerSeek score 70.30

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 832
variable_sites: 29
pi: 0.035
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TCAAAGGTATCATCCAACCGT	AAACGCTTTTAACGCTGTCC	42.9	45.0	57.0	57.6	968-975 bp	1.0	63.6
ndhF-rpl32_p2	TCAAAGGTATCATCCAACCGT	CGCTTTTAACGCTGTCCAAT	42.9	45.0	57.0	57.4	965-972 bp	1.0	61.9
ndhF-rpl32_p3	TCAAAGGTATCATCCAACCGT	AACGCTGTCCAATATCCTCC	42.9	50.0	57.0	57.4	958-965 bp	1.0	61.8
ndhF-rpl32_p4	TCAAAGGTATCATCCAACCGT	AACGCTTTTAACGCTGTCCA	42.9	45.0	57.0	58.7	967-974 bp	1.0	61.2
ndhF-rpl32_p5	TCAAAGGTATCATCCAACCGT	ACGCTTTTAACGCTGTCCAA	42.9	45.0	57.0	58.7	966-973 bp	1.0	61.2