Hotspot

trnR-ACG-trnN-GUU

IRb · 107369-107945 · 577 bp

Back to Scleromitrion

Pi 0.0104

MarkerSeek score 62.70

Primer available yes

Label trnR-ACG-trnN-GUU

Feature Metadata

label_name: trnR-ACG-trnN-GUU
feature_type: igs
region: IRb
length_bp: 577
variable_sites: 6
pi: 0.0104
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnN-GUU_p1	GCGAACAGAACAGATCCAGT	TGGTCGTAGGTTCGAATCCT	50.0	50.0	57.9	58.2	714 bp	1.0	78.7
trnR-ACG-trnN-GUU_p2	GCGAACAGAACAGATCCAGT	CGATTGGTCGTAGGTTCGAA	50.0	50.0	57.9	57.7	718 bp	1.0	77.8
trnR-ACG-trnN-GUU_p3	CAGAGGATTAGAGCACGTGG	TGGTCGTAGGTTCGAATCCT	55.0	50.0	57.8	58.2	670 bp	1.0	77.7
trnR-ACG-trnN-GUU_p4	GCGAACAGAACAGATCCAGT	GGTTCGAATCCTACTTGGGG	50.0	55.0	57.9	57.7	706 bp	1.0	77.4
trnR-ACG-trnN-GUU_p5	GCGAACAGAACAGATCCAGT	TCAGTAGCTCAGCGGTAGAG	50.0	55.0	57.9	58.3	753 bp	1.0	77.3