Hotspot

rps11-rpl36

LSC · 78961-79092 · 132 bp

Back to Scleromitrion

Pi 0.0268

MarkerSeek score 69.40

Primer available yes

Label rps11-rpl36

Feature Metadata

label_name: rps11-rpl36
feature_type: igs
region: LSC
length_bp: 132
variable_sites: 3
pi: 0.0268
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl36_p1	CCGTGTACTCTTAATCCGTCC	AAATAAGAGCTTCGGTGCGT	52.4	45.0	57.9	57.9	282-302 bp	1.0	75.5
rps11-rpl36_p2	CCGTGTACTCTTAATCCGTCC	AGAGCTTCGGTGCGTAAAAT	52.4	45.0	57.9	57.9	277-297 bp	1.0	75.5
rps11-rpl36_p3	TGGAAGCTTGCTTGAACATG	AAATAAGAGCTTCGGTGCGT	45.0	45.0	56.9	57.9	323-343 bp	1.0	75.2
rps11-rpl36_p4	TGGAAGCTTGCTTGAACATG	AGAGCTTCGGTGCGTAAAAT	45.0	45.0	56.9	57.9	318-338 bp	1.0	75.2
rps11-rpl36_p5	CCGTGTACTCTTAATCCGTCC	AAGAGCTTCGGTGCGTAAAA	52.4	45.0	57.9	58.1	278-298 bp	1.0	75.2