Hotspot

petA-psbJ

LSC · 62490-63528 · 1039 bp

Back to Scleromitrion

Pi 0.0221

MarkerSeek score 71.50

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1039
variable_sites: 23
pi: 0.0221
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GTCTCCAAGGCCTTTTGGTC	ATTCCGGATTGGGTTCATCC	55.0	50.0	58.8	57.6	1175-1186 bp	1.0	72.6
petA-psbJ_p2	TGGTCTTCTTGGCATCTGTT	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.0	57.6	1160-1171 bp	1.0	71.0
petA-psbJ_p3	TTGGTCTTCTTGGCATCTGT	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.0	57.6	1161-1172 bp	1.0	71.0
petA-psbJ_p4	GCCTTTTGGTCTTCTTGGCA	ATTCCGGATTGGGTTCATCC	50.0	50.0	59.0	57.6	1166-1177 bp	1.0	71.0
petA-psbJ_p5	TTGGTCTTCTTGGCATCTGTT	ATTCCGGATTGGGTTCATCC	42.9	50.0	57.8	57.6	1161-1172 bp	1.0	68.8