Hotspot

ycf4-cemA

LSC · 59656-60550 · 895 bp

Back to Scleromitrion

Pi 0.0205

MarkerSeek score 69.20

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 895
variable_sites: 18
pi: 0.0205
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TTGGCCTATTTCTTGCGTGT	GAAGCTTTAACGCGGGAGTA	45.0	50.0	58.1	58.0	959-974 bp	1.0	82.0
ycf4-cemA_p2	TTGGCCTATTTCTTGCGTGT	AAGGCACCAGGGCAAAAATA	45.0	45.0	58.1	58.0	988-1003 bp	1.0	81.7
ycf4-cemA_p3	TTGGCCTATTTCTTGCGTGT	CCACCACTTAGTGACCCAAG	45.0	55.0	58.1	57.8	1036-1051 bp	1.0	80.3
ycf4-cemA_p4	TTGGCCTATTTCTTGCGTGT	TCCACCACTTAGTGACCCAA	45.0	50.0	58.1	58.2	1037-1052 bp	1.0	80.2
ycf4-cemA_p5	TTGGCCTATTTCTTGCGTGT	TTCCACCACTTAGTGACCCA	45.0	50.0	58.1	58.2	1038-1053 bp	1.0	80.2