Hotspot

ndhC-trnV-UAC

LSC · 50069-50582 · 514 bp

Back to Scleromitrion

Pi 0.0146

MarkerSeek score 67.70

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 514
variable_sites: 7
pi: 0.0146
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 67.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GGGGCTAAAACTCCCGAAAT	CCGAGAAAGTCTACGGTTCG	50.0	55.0	57.9	58.1	639-648 bp	1.0	82.9
ndhC-trnV-UAC_p2	CCTTTGCTAATCGGGGCTAA	CCGAGAAAGTCTACGGTTCG	50.0	55.0	57.7	58.1	651-660 bp	1.0	81.3
ndhC-trnV-UAC_p3	GGGGCTAAAACTCCCGAAAT	GTTCGAGTCCGTATAGCCCT	50.0	55.0	57.9	58.7	624-633 bp	1.0	78.3
ndhC-trnV-UAC_p4	CCTTTGCTAATCGGGGCTAA	GTTCGAGTCCGTATAGCCCT	50.0	55.0	57.7	58.7	636-645 bp	1.0	76.7
ndhC-trnV-UAC_p5	GGGGCTAAAACTCCCGAAAT	CGAGAAAGTCTACGGTTCGA	50.0	50.0	57.9	57.1	638-647 bp	1.0	76.6