Hotspot

trnS-UGA-psbZ

LSC · 35198-35517 · 320 bp

Back to Scleromitrion

Pi 0.0187

MarkerSeek score 63.20

Primer available yes

Label trnS-UGA-psbZ

Feature Metadata

label_name: trnS-UGA-psbZ
feature_type: igs
region: LSC
length_bp: 320
variable_sites: 6
pi: 0.0187
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbZ_p1	CTATCAACCACTCAGCCACC	AGACAACGGGCACACTAATC	55.0	50.0	58.0	57.9	423-426 bp	1.0	80.1
trnS-UGA-psbZ_p2	CTATCAACCACTCAGCCACC	GTCGGGAGAAGCAAAGACAA	55.0	50.0	58.0	58.1	437-440 bp	1.0	79.9
trnS-UGA-psbZ_p3	CTATCAACCACTCAGCCACC	TCGGGAGAAGCAAAGACAAC	55.0	50.0	58.0	58.1	436-439 bp	1.0	79.9
trnS-UGA-psbZ_p4	CTATCAACCACTCAGCCACC	TGTTACTTGACCAACCGTCG	55.0	50.0	58.0	58.1	453-456 bp	1.0	79.7
trnS-UGA-psbZ_p5	GTTTTCAAGACCGGGGCTAT	AGACAACGGGCACACTAATC	50.0	50.0	57.9	57.9	439-442 bp	1.0	79.3