Hotspot

atpH-atpI

LSC · 13368-14488 · 1121 bp

Back to Scleromitrion

Pi 0.0213

MarkerSeek score 61.10

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1121
variable_sites: 23
pi: 0.0213
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	CTCTACAGCTTGACCTGCAG	ACGCAGGTCTTACCAAAAGG	55.0	50.0	58.0	58.1	1508-1528 bp	1.0	82.5
atpH-atpI_p2	CTCTACAGCTTGACCTGCAG	TGCTTTGGCTTTACTCACGT	55.0	45.0	58.0	58.0	1563 bp	0.5	59.6
atpH-atpI_p3	CAAACCAGCAGCAATAACGG	TGCTTTGGCTTTACTCACGT	50.0	45.0	58.0	58.0	1497 bp	0.5	59.5
atpH-atpI_p4	CCAAACCAGCAGCAATAACG	TGCTTTGGCTTTACTCACGT	50.0	45.0	58.0	58.0	1498 bp	0.5	59.5
atpH-atpI_p5	AGTCGGGTCGGTATACCAAT	TGCTTTGGCTTTACTCACGT	50.0	45.0	57.9	58.0	1863 bp	0.5	59.1