Hotspot

trnK-UUU-rps16

LSC · 4119-4864 · 746 bp

Back to Scleromitrion

Pi 0.0242

MarkerSeek score 59.20

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 746
variable_sites: 18
pi: 0.0242
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CTCTCCTTCAACCCGACATG	TTTCCTTGAAAGGGGTGCTC	55.0	50.0	58.0	58.0	1008-1011 bp	1.0	81.8
trnK-UUU-rps16_p2	CGTGAATACACTCATGGGCA	TTTCCTTGAAAGGGGTGCTC	50.0	50.0	58.0	58.0	1248-1251 bp	1.0	81.7
trnK-UUU-rps16_p3	CTCTCCTTCAACCCGACATG	CTTGAAAGGGGTGCTCAACT	55.0	50.0	58.0	58.0	1004-1007 bp	1.0	81.7
trnK-UUU-rps16_p4	CGTGAATACACTCATGGGCA	CTTGAAAGGGGTGCTCAACT	50.0	50.0	58.0	58.0	1244-1247 bp	1.0	81.6
trnK-UUU-rps16_p5	CTCTCCTTCAACCCGACATG	AGCTCTTCAAAACGTGGGTT	55.0	45.0	58.0	57.9	1080-1083 bp	1.0	81.3