Hotspot

psbA-trnK-UUU

LSC · 1385-1581 · 197 bp

Back to Scleromitrion

Pi 0.0203

MarkerSeek score 64.10

Primer available yes

Label psbA-trnK-UUU

Feature Metadata

label_name: psbA-trnK-UUU
feature_type: igs
region: LSC
length_bp: 197
variable_sites: 4
pi: 0.0203
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	TTGCAGAAACGACCCCATAG	GGAGGGGTGTTTCCTTGTTT	50.0	50.0	57.9	57.9	336-346 bp	1.0	79.4
psbA-trnK-UUU_p2	AAACGACCCCATAGGCTTTC	GGAGGGGTGTTTCCTTGTTT	50.0	50.0	57.9	57.9	330-340 bp	1.0	79.3
psbA-trnK-UUU_p3	GAAACGACCCCATAGGCTTT	GGAGGGGTGTTTCCTTGTTT	50.0	50.0	57.9	57.9	331-341 bp	1.0	79.3
psbA-trnK-UUU_p4	GTTGCAGAAACGACCCCATA	GGAGGGGTGTTTCCTTGTTT	50.0	50.0	58.2	57.9	337-347 bp	1.0	78.8
psbA-trnK-UUU_p5	TTGCAGAAACGACCCCATAG	CTAGTTCCGGGTTCGAATCC	50.0	55.0	57.9	57.8	284-294 bp	1.0	78.5