Hotspot

ycf1

SSC · 122385-127856 · 5472 bp

Pi 0.0314

MarkerSeek score 57.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5472
variable_sites: 171
pi: 0.0314
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AGATTTTTCTTGACTTTCTGAATCA	TCGACAGCGGATTCTGTAGA	28.0	50.0	55.4	58.3	207-213 bp	1.0	46.9
ycf1_p2	TCAAAGATTTTTCTTGACTTTCTGA	CGACAGCGGATTCTGTAGAA	28.0	50.0	55.6	57.4	210-216 bp	1.0	46.8
ycf1_p3	TCAAAGATTTTTCTTGACTTTCTGA	TCGACAGCGGATTCTGTAGA	28.0	50.0	55.6	58.3	211-217 bp	1.0	46.7
ycf1_p4	AGATTTTTCTTGACTTTCTGAATCA	AACTTCGACAGCGGATTCTG	28.0	50.0	55.4	58.3	211-217 bp	1.0	46.7
ycf1_p5	TCAAAGATTTTTCTTGACTTTCTGA	AACTTCGACAGCGGATTCTG	28.0	50.0	55.6	58.3	215-221 bp	1.0	46.6