Hotspot

trnL-UAG-ccsA

SSC · 112818-112895 · 78 bp

Back to Scilla

Pi 0.0685
MarkerSeek score 79.40
Primer available yes
Label trnL-UAG-ccsA

Feature Metadata

label_name
trnL-UAG-ccsA
feature_type
igs
region
SSC
length_bp
78
variable_sites
5
pi
0.0685
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-ccsA_p1 TAGACACGCTGCTCTTAGGA TGATCGAAAAAGAGAGATGAGT 50.0 36.4 57.9 55.0 191 bp 1.0 44.2
trnL-UAG-ccsA_p2 GTAGACACGCTGCTCTTAGG TGATCGAAAAAGAGAGATGAGT 55.0 36.4 57.8 55.0 192 bp 1.0 44.2
trnL-UAG-ccsA_p3 GGTAGACACGCTGCTCTTAG TGATCGAAAAAGAGAGATGAGT 55.0 36.4 57.8 55.0 193 bp 1.0 44.1
trnL-UAG-ccsA_p4 AAATTGGTAGACACGCTGCT TGATCGAAAAAGAGAGATGAGT 45.0 36.4 57.8 55.0 198 bp 1.0 44.0
trnL-UAG-ccsA_p5 TTTCAAGTCAGCCGCTATGG TGATCGAAAAAGAGAGATGAGT 50.0 36.4 58.3 55.0 220 bp 1.0 43.6