Hotspot

rpl14-rpl16

LSC · 79547-79695 · 149 bp

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Pi 0.0429
MarkerSeek score 77.40
Primer available yes
Label rpl14-rpl16

Feature Metadata

label_name
rpl14-rpl16
feature_type
igs
region
LSC
length_bp
149
variable_sites
6
pi
0.0429
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
77.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl14-rpl16_p1 ATCAATTTTCGAGCTCCGCT GGGCGGAGTATCAGAAACTG 45.0 55.0 58.0 58.1 290-299 bp 1.0 83.4
rpl14-rpl16_p2 TTTCGAGCTCCGCTGTTATC GGGCGGAGTATCAGAAACTG 50.0 55.0 58.1 58.1 284-293 bp 1.0 83.1
rpl14-rpl16_p3 CATCAATTTTCGAGCTCCGC GGGCGGAGTATCAGAAACTG 50.0 55.0 57.9 58.1 291-300 bp 1.0 82.8
rpl14-rpl16_p4 ATCAATTTTCGAGCTCCGCT GCTGCATGCAAAATGCCTAT 45.0 45.0 58.0 57.8 247-256 bp 1.0 82.7
rpl14-rpl16_p5 ATCAATTTTCGAGCTCCGCT ATAGCTGCATGCAAAATGCC 45.0 45.0 58.0 57.8 250-259 bp 1.0 82.6