Hotspot

trnW-CCA-trnP-UGG

LSC · 64909-65081 · 173 bp

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Pi 0.0539
MarkerSeek score 78.90
Primer available yes
Label trnW-CCA-trnP-UGG

Feature Metadata

label_name
trnW-CCA-trnP-UGG
feature_type
igs
region
LSC
length_bp
173
variable_sites
9
pi
0.0539
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnW-CCA-trnP-UGG_p1 GCGTTCTACCGAACTGAACT CACGGGTTCAAATCCTGTCA 50.0 50.0 57.9 58.1 223-227 bp 1.0 84.0
trnW-CCA-trnP-UGG_p2 GCGTTCTACCGAACTGAACT GTCACGGGTTCAAATCCTGT 50.0 50.0 57.9 58.1 225-229 bp 1.0 83.9
trnW-CCA-trnP-UGG_p3 GCGTTCTACCGAACTGAACT TGTCACGGGTTCAAATCCTG 50.0 50.0 57.9 58.1 226-230 bp 1.0 83.9
trnW-CCA-trnP-UGG_p4 GCGTTCTACCGAACTGAACT GGGTACAAAATGTCACGGGT 50.0 50.0 57.9 58.1 236-240 bp 1.0 83.8
trnW-CCA-trnP-UGG_p5 GCGTTCTACCGAACTGAACT TGGGTACAAAATGTCACGGG 50.0 50.0 57.9 58.1 237-241 bp 1.0 83.7