Hotspot

petA-psbJ

LSC · 61643-62255 · 613 bp

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Pi 0.0425
MarkerSeek score 77.90
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
613
variable_sites
26
pi
0.0425
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 ATCCATTACGTGTCCAAGGC GGTTCATATTCCGGGTTGGG 50.0 55.0 58.0 58.3 765-942 bp 1.0 79.7
petA-psbJ_p2 ATCCATTACGTGTCCAAGGC CATATTCCGGGTTGGGTTCA 50.0 50.0 58.0 57.6 761-938 bp 1.0 78.8
petA-psbJ_p3 ATCCATTACGTGTCCAAGGC GTTCATATTCCGGGTTGGGT 50.0 50.0 58.0 57.6 764-941 bp 1.0 78.8
petA-psbJ_p4 CATTACGTGTCCAAGGCCTT GGTTCATATTCCGGGTTGGG 50.0 55.0 58.2 58.3 762-939 bp 1.0 78.6
petA-psbJ_p5 CATTACGTGTCCAAGGCCTT CATATTCCGGGTTGGGTTCA 50.0 50.0 58.2 57.6 758-935 bp 1.0 77.7