Hotspot

atpB-rbcL

LSC · 53712-54507 · 796 bp

Back to Scilla

Pi 0.0506
MarkerSeek score 73.80
Primer available yes
Label atpB-rbcL

Feature Metadata

label_name
atpB-rbcL
feature_type
igs
region
LSC
length_bp
796
variable_sites
40
pi
0.0506
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
73.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpB-rbcL_p1 GACCGGAACACTTAGAGCAG GTCCACACAGTTGTCCATGT 55.0 50.0 58.0 58.0 1314-1332 bp 1.0 83.0
atpB-rbcL_p2 GACCGGAACACTTAGAGCAG CGGTCTCTCCAACGCATAAA 55.0 50.0 58.0 58.0 1752-1770 bp 1.0 82.8
atpB-rbcL_p3 GAACACTTAGAGCAGCTCCC CGGTCTCTCCAACGCATAAA 55.0 50.0 58.0 58.0 1747-1765 bp 1.0 82.7
atpB-rbcL_p4 GACCGGAACACTTAGAGCAG ATCCTCCAGACGTAGAGCTC 55.0 55.0 58.0 58.0 1513-1531 bp 1.0 82.7
atpB-rbcL_p5 GGAACACTTAGAGCAGCTCC CGGTCTCTCCAACGCATAAA 55.0 50.0 58.0 58.0 1748-1766 bp 1.0 82.6