Hotspot

trnM-CAU-atpE

LSC · 51649-51822 · 174 bp

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Pi 0.0632
MarkerSeek score 73.20
Primer available yes
Label trnM-CAU-atpE

Feature Metadata

label_name
trnM-CAU-atpE
feature_type
igs
region
LSC
length_bp
174
variable_sites
11
pi
0.0632
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnM-CAU-atpE_p1 CAGAGGCGATGGGTTCTTTT CTCTCAGACGAGCTAGGACA 50.0 55.0 58.2 58.0 336-340 bp 1.0 81.0
trnM-CAU-atpE_p2 CAGAGGCGATGGGTTCTTTT TAGGACACGAGTAGAGGCTG 50.0 55.0 58.2 58.0 323-327 bp 1.0 81.0
trnM-CAU-atpE_p3 CAGAGGCGATGGGTTCTTTT GCGAATCTAGCTCTCAGACG 50.0 55.0 58.2 58.0 346-350 bp 1.0 80.9
trnM-CAU-atpE_p4 CAGAGGCGATGGGTTCTTTT CGAGCTAGGACACGAGTAGA 50.0 55.0 58.2 58.1 328-332 bp 1.0 80.8
trnM-CAU-atpE_p5 CAGAGGCGATGGGTTCTTTT TGAGGCGAATCTAGCTCTCA 50.0 50.0 58.2 57.9 350-354 bp 1.0 80.6