Hotspot

ndhC-trnV-UAC

LSC · 49772-50746 · 975 bp

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Pi 0.0432
MarkerSeek score 71.60
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
975
variable_sites
41
pi
0.0432
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
71.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TTTCTGGCCCTTCGCTAATC GGGAGCCCATTATGCAATGA 50.0 50.0 58.0 58.0 1705-1957 bp 1.0 83.1
ndhC-trnV-UAC_p2 TTTCTGGCCCTTCGCTAATC AGGGAGCCCATTATGCAATG 50.0 50.0 58.0 58.0 1706-1958 bp 1.0 83.1
ndhC-trnV-UAC_p3 CGCTAATCGGGGCTAAAACT GGGAGCCCATTATGCAATGA 50.0 50.0 58.1 58.0 1693-1945 bp 1.0 83.0
ndhC-trnV-UAC_p4 TAGAGAGCTTTTCTGGCCCT GGGAGCCCATTATGCAATGA 50.0 50.0 58.0 58.0 1714 bp 0.5 59.7
ndhC-trnV-UAC_p5 TAGAGAGCTTTTCTGGCCCT AGGGAGCCCATTATGCAATG 50.0 50.0 58.0 58.0 1715 bp 0.5 59.6