Hotspot

trnT-GGU-psbD

LSC · 31578-32656 · 1079 bp

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Pi 0.0362
MarkerSeek score 65.50
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1079
variable_sites
39
pi
0.0362
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GCATCGACCCAGGAAGAATT TCGCAGTTGAACAGATCGAG 50.0 50.0 58.0 58.0 2201-2212 bp 1.0 82.6
trnT-GGU-psbD_p2 GCATCGACCCAGGAAGAATT CTGCTACACCAAAACTGGGT 50.0 50.0 58.0 58.0 2313-2324 bp 1.0 82.4
trnT-GGU-psbD_p3 GCATCGACCCAGGAAGAATT CACCGTCACCATCTTCGAAT 50.0 50.0 58.0 58.0 2469-2480 bp 1.0 82.3
trnT-GGU-psbD_p4 GTGGTAGAGTAACGCCATGG CACCGTCACCATCTTCGAAT 55.0 50.0 58.1 58.0 1825-1837 bp 1.0 81.5
trnT-GGU-psbD_p5 GCATCGACCCAGGAAGAATT TGGCAAATTTACCAAGGGCT 50.0 45.0 58.0 58.0 1809 bp 0.5 59.5