Hotspot

trnY-GUA-trnE-UUC

LSC · 30886-30944 · 59 bp

Back to Scilla

Pi 0.0508
MarkerSeek score 75.40
Primer available yes
Label trnY-GUA-trnE-UUC

Feature Metadata

label_name
trnY-GUA-trnE-UUC
feature_type
igs
region
LSC
length_bp
59
variable_sites
3
pi
0.0508
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
75.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnY-GUA-trnE-UUC_p1 GACTCATGGAGCGGTGAATT AAGGAGAAAACGGGGATTCG 50.0 50.0 58.0 57.9 201-205 bp 1.0 80.1
trnY-GUA-trnE-UUC_p2 TATCAGACTCATGGAGCGGT AAGGAGAAAACGGGGATTCG 50.0 50.0 57.9 57.9 206-210 bp 1.0 79.7
trnY-GUA-trnE-UUC_p3 GACTCATGGAGCGGTGAATT AAAACGGGGATTCGACTTCC 50.0 50.0 58.0 58.2 195-199 bp 1.0 79.5
trnY-GUA-trnE-UUC_p4 TATCAGACTCATGGAGCGGT AAAACGGGGATTCGACTTCC 50.0 50.0 57.9 58.2 200-204 bp 1.0 79.1
trnY-GUA-trnE-UUC_p5 GACTCATGGAGCGGTGAATT ATATGCCCCCATCGTCTAGT 50.0 50.0 58.0 57.7 242-246 bp 1.0 78.1