Hotspot

psbM-trnD-GUC

LSC · 29724-30409 · 686 bp

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Pi 0.0838
MarkerSeek score 79.10
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
686
variable_sites
57
pi
0.0838
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
79.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 TGCGAGAATATTGACTTCCA TTCAATTGGTCAGAGCACCG 40.0 50.0 54.5 58.5 771-934 bp 1.0 53.9
psbM-trnD-GUC_p2 TGCGAGAATATTGACTTCCAT TTCAATTGGTCAGAGCACCG 38.1 50.0 55.1 58.5 771-934 bp 1.0 50.9
psbM-trnD-GUC_p3 TGCGAGAATATTGACTTCCA GTTCAATTGGTCAGAGCACC 40.0 50.0 54.5 57.0 772-935 bp 1.0 49.8
psbM-trnD-GUC_p4 TGCGAGAATATTGACTTCCA TCAATTGGTCAGAGCACCG 40.0 52.6 54.5 57.8 770-933 bp 1.0 47.9
psbM-trnD-GUC_p5 TGCGAGAATATTGACTTCCAT GTTCAATTGGTCAGAGCACC 38.1 50.0 55.1 57.0 772-935 bp 1.0 46.7