Hotspot

rpoB-trnC-GCA

LSC · 26458-27659 · 1202 bp

Back to Scilla

Pi 0.0285
MarkerSeek score 68.00
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1202
variable_sites
33
pi
0.0285
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 GTGGACATTCCCTCATTTCCA TAAAGGATTTGCAGTCCCCC 47.6 50.0 57.9 57.5 1278-1310 bp 1.0 71.8
rpoB-trnC-GCA_p2 TGTGGACATTCCCTCATTTCC TAAAGGATTTGCAGTCCCCC 47.6 50.0 57.9 57.5 1279-1311 bp 1.0 71.8
rpoB-trnC-GCA_p3 GTGGACATTCCCTCATTTCCA TTTGTTGATCAGGCGACACC 47.6 50.0 57.9 58.8 1315-1347 bp 1.0 69.8
rpoB-trnC-GCA_p4 TGTGGACATTCCCTCATTTCC TTTGTTGATCAGGCGACACC 47.6 50.0 57.9 58.8 1316-1348 bp 1.0 69.8
rpoB-trnC-GCA_p5 CCAGGTATTGTGGACATTCCC TAAAGGATTTGCAGTCCCCC 52.4 50.0 58.3 57.5 1287-1319 bp 1.0 69.7