Hotspot

trnS-GCU-trnG-UCC

LSC · 8061-9032 · 972 bp

Back to Scilla

Pi 0.0507
MarkerSeek score 79.00
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
972
variable_sites
47
pi
0.0507
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
79.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 GCTTTAGTCCACTCAGCCAT TGAACGAATCACACTTTTACCAC 50.0 39.1 57.6 57.6 1035-1085 bp 1.0 56.4
trnS-GCU-trnG-UCC_p2 AGTCCACTCAGCCATCTCTC TGAACGAATCACACTTTTACCAC 55.0 39.1 58.5 57.6 1030-1080 bp 1.0 55.5
trnS-GCU-trnG-UCC_p3 GCTTTAGTCCACTCAGCCAT ACGAATCACACTTTTACCACT 50.0 38.1 57.6 55.5 1032-1082 bp 1.0 55.4
trnS-GCU-trnG-UCC_p4 AGTCCACTCAGCCATCTCTC ACGAATCACACTTTTACCACT 55.0 38.1 58.5 55.5 1027-1077 bp 1.0 54.6
trnS-GCU-trnG-UCC_p5 GCTTTAGTCCACTCAGCCAT GAACGAATCACACTTTTACCACT 50.0 39.1 57.6 57.3 1034-1084 bp 1.0 54.3