Hotspot

rps16-trnQ-UUG

LSC · 5809-6732 · 924 bp

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Pi 0.0418
MarkerSeek score 64.20
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
924
variable_sites
19
pi
0.0418
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.49
markerseek_score
64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 TATGTCCTTCAAGTCGCACG GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 557-1007 bp 1.0 85.3
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 547-997 bp 1.0 84.2
rps16-trnQ-UUG_p3 TATGTCCTTCAAGTCGCACG CTCGGAGGTTCGAATCCTTC 50.0 55.0 58.0 57.8 561-1011 bp 1.0 84.2
rps16-trnQ-UUG_p4 AAGTCGCACGTTGCTTTCTA CTCGGAGGTTCGAATCCTTC 45.0 55.0 58.1 57.8 551-1001 bp 1.0 83.2
rps16-trnQ-UUG_p5 TATGTCCTTCAAGTCGCACG TACTCGGAGGTTCGAATCCT 50.0 50.0 58.0 57.6 563-1013 bp 1.0 82.4