Hotspot

trnK-UUU-rps16

LSC · 4055-4693 · 639 bp

Back to Scilla

Pi 0.046
MarkerSeek score 71.90
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
639
variable_sites
29
pi
0.046
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
71.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AGTCGCACTTAAAAGCCGAA TCGATCCCGAAGAGAAGGAA 45.0 50.0 58.1 57.9 863-875 bp 1.0 81.6
trnK-UUU-rps16_p2 AGTCGCACTTAAAAGCCGAA GCATGTTGCTGCCTTTTGAA 45.0 45.0 58.1 58.1 1336-1353 bp 1.0 81.1
trnK-UUU-rps16_p3 TCCTCTCCCTCCCCTTCTAT GCATGTTGCTGCCTTTTGAA 55.0 45.0 58.1 58.1 1937 bp 0.5 58.5
trnK-UUU-rps16_p4 TCCTCTCCCTCCCCTTCTAT TCGATCCCGAAGAGAAGGAA 55.0 50.0 58.1 57.9 1464 bp 0.5 58.4
trnK-UUU-rps16_p5 TCCTCTCCCTCCCCTTCTAT GGTGCTCAACCTACAGGAAC 55.0 55.0 58.1 58.2 1358 bp 0.5 58.0