Hotspot

ycf1

SSC · 122998-128532 · 5535 bp

Back to Schweinfurthia

Pi 0.000181

MarkerSeek score 52.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5535
variable_sites: 1
pi: 0.000181
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCAAGTGTAGTGGGGGATAGT	CCCCTTTTGTCTCTTCTTTTCG	47.6	45.5	57.5	57.5	218 bp	1.0	48.6
ycf1_p2	AGTGTAGTGGGGGATAGTCT	CCCCTTTTGTCTCTTCTTTTCG	50.0	45.5	56.4	57.5	215 bp	1.0	48.0
ycf1_p3	TTTTCAAGTGTAGTGGGGGA	CCCCTTTTGTCTCTTCTTTTCG	45.0	45.5	56.3	57.5	221 bp	1.0	46.6
ycf1_p4	AAGTGTAGTGGGGGATAGTCT	CCCCTTTTGTCTCTTCTTTTCG	47.6	45.5	57.2	57.5	216 bp	1.0	46.2
ycf1_p5	AGTGTAGTGGGGGATAGTCTT	CCCCTTTTGTCTCTTCTTTTCG	47.6	45.5	57.2	57.5	215 bp	1.0	46.2