Hotspot

rps19

LSC · 83439-83717 · 279 bp

Back to Schweinfurthia

Pi 0.0108

MarkerSeek score 58.70

Primer available yes

Label rps19

Feature Metadata

label_name: rps19
feature_type: gene
region: LSC
length_bp: 279
variable_sites: 3
pi: 0.0108
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19_p1	CGACCTAAAGCGTATACTTCTGT	GATTGTTCGTCGCCGTAGTA	43.5	50.0	58.1	57.8	464-465 bp	1.0	54.6
rps19_p2	CGACCTAAAGCGTATACTTCTGT	TTTGATTGTTCGTCGCCGTA	43.5	45.0	58.1	58.2	467-468 bp	1.0	54.3
rps19_p3	CGACCTAAAGCGTATACTTCTGT	AATTTGATTGTTCGTCGCCG	43.5	45.0	58.1	57.8	469-470 bp	1.0	54.1
rps19_p4	TCGACCTAAAGCGTATACTTCTG	GATTGTTCGTCGCCGTAGTA	43.5	50.0	57.8	57.8	465-466 bp	1.0	53.9
rps19_p5	TCGACCTAAAGCGTATACTTCTG	TTTGATTGTTCGTCGCCGTA	43.5	45.0	57.8	58.2	468-469 bp	1.0	53.6