Hotspot

rps16

LSC · 4911-6078 · 1168 bp

Back to Schweinfurthia

Pi 0.000856

MarkerSeek score 59.20

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1168
variable_sites: 1
pi: 0.000856
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	TCTATACAAGTTGCTACCCCC	TGTACAACCGAACCAATGACT	47.6	42.9	56.5	57.5	1279 bp	1.0	48.3
rps16_p2	TTCTATACAAGTTGCTACCCCC	TGTACAACCGAACCAATGACT	45.5	42.9	57.2	57.5	1280 bp	1.0	46.1
rps16_p3	TCTATACAAGTTGCTACCCCC	TGTACAACCGAACCAATGAC	47.6	45.0	56.5	56.0	1279 bp	1.0	44.1
rps16_p4	TCTATACAAGTTGCTACCCCC	ATGTACAACCGAACCAATGACT	47.6	40.9	56.5	58.0	1280 bp	1.0	44.0
rps16_p5	CTATACAAGTTGCTACCCCC	TGTACAACCGAACCAATGACT	50.0	42.9	54.9	57.5	1278 bp	1.0	43.6