Hotspot

psbA

LSC · 310-1368 · 1059 bp

Back to Schweinfurthia

Pi 0

MarkerSeek score 40.00

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1059
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	GGAGCAATAACCCCTCTTGA	AAATTCGTGTGCTTGGGAGT	50.0	45.0	56.9	58.0	1207 bp	1.0	71.0
psbA_p2	AGGAGCAATAACCCCTCTTG	AAATTCGTGTGCTTGGGAGT	50.0	45.0	56.9	58.0	1208 bp	1.0	71.0
psbA_p3	AAGGAGCAATAACCCCTCTTG	AAATTCGTGTGCTTGGGAGT	47.6	45.0	57.6	58.0	1209 bp	1.0	68.9
psbA_p4	AGGAGCAATAACCCCTCTTGA	AAATTCGTGTGCTTGGGAGT	47.6	45.0	58.4	58.0	1208 bp	1.0	68.3
psbA_p5	GGAGCAATAACCCCTCTTGA	AATTCGTGTGCTTGGGAGTC	50.0	50.0	56.9	58.5	1206 bp	1.0	67.4