Hotspot

atpF

LSC · 11539-12789 · 1251 bp

Back to Schweinfurthia

Pi 0

MarkerSeek score 58.90

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1251
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	GGCTCGAATGGTTACCATGA	AAGAGGGGCAAAGTAATAGA	50.0	40.0	57.7	53.3	1397-1399 bp	1.0	40.1
atpF_p2	GGCTCGAATGGTTACCATGA	AGAGGGGCAAAGTAATAGAA	50.0	40.0	57.7	53.3	1396-1398 bp	1.0	40.1
atpF_p3	GGCTCGAATGGTTACCATGA	AAAGAGGGGCAAAGTAATAGA	50.0	38.1	57.7	54.2	1398-1400 bp	1.0	40.1
atpF_p4	GGCTCGAATGGTTACCATGA	AAGAGGGGCAAAGTAATAGAA	50.0	38.1	57.7	54.2	1397-1399 bp	1.0	40.1
atpF_p5	GGCTCGAATGGTTACCATGA	AGAGGGGCAAAGTAATAGAAA	50.0	38.1	57.7	54.2	1396-1398 bp	1.0	40.1