Hotspot

ccsA-ndhD

SSC · 114587-114831 · 245 bp

Back to Schweinfurthia

Pi 0

MarkerSeek score 19.30

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 245
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 19.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TTTTTACCCAAGGCTTCGCT	TCAGTAGCTTTTCGATGGCC	45.0	50.0	58.0	58.0	1269 bp	1.0	79.8
ccsA-ndhD_p2	TTTTTACCCAAGGCTTCGCT	CAGTAGCTTTTCGATGGCCT	45.0	50.0	58.0	58.0	1268 bp	1.0	79.7
ccsA-ndhD_p3	AATTCAGCAATTGTGGCGTG	TCAGTAGCTTTTCGATGGCC	45.0	50.0	57.9	58.0	733 bp	1.0	79.4
ccsA-ndhD_p4	AATTCAGCAATTGTGGCGTG	CAGTAGCTTTTCGATGGCCT	45.0	50.0	57.9	58.0	732 bp	1.0	79.3
ccsA-ndhD_p5	GGAAGGGTCCAAATTCAGCA	TCAGTAGCTTTTCGATGGCC	50.0	50.0	58.1	58.0	744 bp	1.0	79.3