Hotspot

rpl22-rps19

LSC · 83377-83438 · 62 bp

Back to Schweinfurthia

Pi 0
MarkerSeek score 40.10
Primer available yes
Label rpl22-rps19

Feature Metadata

label_name
rpl22-rps19
feature_type
igs
region
LSC
length_bp
62
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
40.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl22-rps19_p1 AATTTCTTCGCCGTAGTCCC AAAAGTTTGGGTAGAGCCGG 50.0 50.0 58.0 58.1 895-896 bp 1.0 79.4
rpl22-rps19_p2 CGCATAACTACGTCCTCGAG AAAAGTTTGGGTAGAGCCGG 55.0 50.0 58.0 58.1 926-927 bp 1.0 79.4
rpl22-rps19_p3 AATTTCTTCGCCGTAGTCCC ATTACCTTCTGGGGAGGTCC 50.0 55.0 58.0 58.1 977-978 bp 1.0 79.3
rpl22-rps19_p4 CGCATAACTACGTCCTCGAG ATTACCTTCTGGGGAGGTCC 55.0 55.0 58.0 58.1 1008-1009 bp 1.0 79.3
rpl22-rps19_p5 AATTTCTTCGCCGTAGTCCC TGGGGAGGTCCGTTTGATAT 50.0 50.0 58.0 58.1 968-969 bp 1.0 79.3