Hotspot

rps18-rpl20

LSC · 67928-68167 · 240 bp

Back to Schweinfurthia

Pi 0
MarkerSeek score 52.90
Primer available yes
Label rps18-rpl20

Feature Metadata

label_name
rps18-rpl20
feature_type
igs
region
LSC
length_bp
240
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps18-rpl20_p1 AACCGAGTCGACCACTAGAA CCAACGAAATCCGAAAAGAAGT 50.0 40.9 58.1 57.5 341-342 bp 1.0 59.6
rps18-rpl20_p2 AAGAACCGAGTCGACCACTA CCAACGAAATCCGAAAAGAAGT 50.0 40.9 58.1 57.5 344-345 bp 1.0 59.6
rps18-rpl20_p3 TTGAAAGAACCGAGTCGACC CCAACGAAATCCGAAAAGAAGT 50.0 40.9 57.9 57.5 348-349 bp 1.0 59.2
rps18-rpl20_p4 AACCGAGTCGACCACTAGAA TCCAACGAAATCCGAAAAGA 50.0 40.0 58.1 55.3 342-343 bp 1.0 57.9
rps18-rpl20_p5 AAGAACCGAGTCGACCACTA TCCAACGAAATCCGAAAAGA 50.0 40.0 58.1 55.3 345-346 bp 1.0 57.9