Hotspot

rps16-trnQ-UUG

LSC · 6079-6816 · 738 bp

Back to Schweinfurthia

Pi 0.00136

MarkerSeek score 58.30

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 738
variable_sites: 1
pi: 0.00136
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	CCACAACGGATCATGTCCTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.9	58.1	832 bp	1.0	78.6
rps16-trnQ-UUG_p2	CCACAACGGATCATGTCCTT	CGAAATTGAAATGGGGCGTC	50.0	50.0	57.9	58.1	891 bp	1.0	78.4
rps16-trnQ-UUG_p3	CCACAACGGATCATGTCCTT	GAAATTGAAATGGGGCGTCG	50.0	50.0	57.9	58.1	890 bp	1.0	78.4
rps16-trnQ-UUG_p4	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	811 bp	1.0	78.4
rps16-trnQ-UUG_p5	AAGTCGCACGTTGCTTTCTA	GAAATTGAAATGGGGCGTCG	45.0	50.0	58.1	58.1	869 bp	1.0	78.2