Hotspot

ycf1

SSC · 125621-131257 · 5637 bp

Back to Schrebera

Pi 0.0105

MarkerSeek score 39.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5637
variable_sites: 127
pi: 0.0105
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 39.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGTCATTACACAACCTAGTCCT	AGAAGGATATCTCTGCCTACACT	40.9	43.5	56.5	57.9	184 bp	1.0	45.7
ycf1_p2	TGTCATTACACAACCTAGTCCT	AGAAGGATATCTCTGCCTACAC	40.9	45.5	56.5	56.5	184 bp	1.0	45.7
ycf1_p3	TGTCATTACACAACCTAGTCCT	GAAGGATATCTCTGCCTACACT	40.9	45.5	56.5	56.5	183 bp	1.0	45.7
ycf1_p4	TGTCATTACACAACCTAGTCC	AGAAGGATATCTCTGCCTACACT	42.9	43.5	55.0	57.9	184 bp	1.0	45.7
ycf1_p5	TTGTCATTACACAACCTAGTCCT	AGAAGGATATCTCTGCCTACACT	39.1	43.5	57.2	57.9	185 bp	1.0	45.6