Hotspot

psaC-ndhE

SSC · 119269-119525 · 257 bp

Back to Schrebera

Pi 0.0127

MarkerSeek score 56.40

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 257
variable_sites: 7
pi: 0.0127
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	GCTCGGACACATTGAGTACA	GCTATTGGACCGGCTATTGT	50.0	50.0	57.6	57.7	391-400 bp	1.0	78.5
psaC-ndhE_p2	GCTCGGACACATTGAGTACA	AGCTATTGGACCGGCTATTG	50.0	50.0	57.6	57.4	392-401 bp	1.0	76.1
psaC-ndhE_p3	GCTCGGACACATTGAGTACA	CAGCTATTGGACCGGCTATT	50.0	50.0	57.6	57.4	393-402 bp	1.0	76.1
psaC-ndhE_p4	GCTCGGACACATTGAGTACA	TTGGACCGGCTATTGTTTCA	50.0	45.0	57.6	57.4	387-396 bp	1.0	76.0
psaC-ndhE_p5	CGCTCGGACACATTGAGTAC	GCTATTGGACCGGCTATTGT	55.0	50.0	58.7	57.7	392-401 bp	1.0	75.5