Hotspot

ndhF-rpl32

SSC · 114460-114932 · 473 bp

Back to Schrebera

Pi 0.0163

MarkerSeek score 47.70

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 473
variable_sites: 16
pi: 0.0163
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GTTGCCGTCGGAAAAAGAAG	ATGCTTTTAACGCTGTCCCA	50.0	45.0	58.0	58.1	663-675 bp	1.0	81.5
ndhF-rpl32_p2	GTTGCCGTCGGAAAAAGAAG	TGCTTTTAACGCTGTCCCAT	50.0	45.0	58.0	58.1	662-674 bp	1.0	81.5
ndhF-rpl32_p3	GTTGCCGTCGGAAAAAGAAG	TAACGCTGTCCCATATCCCT	50.0	50.0	58.0	57.9	656-668 bp	1.0	81.5
ndhF-rpl32_p4	TGCCGTCGGAAAAAGAAGAA	ATGCTTTTAACGCTGTCCCA	45.0	45.0	58.0	58.1	661-673 bp	1.0	81.4
ndhF-rpl32_p5	TGCCGTCGGAAAAAGAAGAA	TGCTTTTAACGCTGTCCCAT	45.0	45.0	58.0	58.1	660-672 bp	1.0	81.4