Hotspot

psbE-petL

LSC · 67191-68355 · 1165 bp

Back to Schrebera

Pi 0.0113

MarkerSeek score 57.60

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1165
variable_sites: 28
pi: 0.0113
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	TGACAAATAACCAACCCGCA	GTCGTATCTTGCTCAGACCA	45.0	50.0	58.0	57.1	1368-1379 bp	1.0	74.0
psbE-petL_p2	CAACCCGCAATGAATAGGGA	GTCGTATCTTGCTCAGACCA	50.0	50.0	57.9	57.1	1357-1368 bp	1.0	73.7
psbE-petL_p3	AGAACGTTCTCCTGTGCTTC	GTCGTATCTTGCTCAGACCA	50.0	50.0	57.8	57.1	1280-1291 bp	1.0	73.0
psbE-petL_p4	GTTCTCCTGTGCTTCCAGAC	GTCGTATCTTGCTCAGACCA	55.0	50.0	58.2	57.1	1275-1286 bp	1.0	72.6
psbE-petL_p5	ACCAACCCGCAATGAATAGG	GTCGTATCTTGCTCAGACCA	50.0	50.0	58.2	57.1	1359-1370 bp	1.0	72.2