Pi
0.0095
MarkerSeek score
51.20
Primer available
yes
Label
trnM(CAU)-trnL(UAA)
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnM(CAU)-trnL(UAA)_p1 | TGAAGGAAAAGCCGGCTATC | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 58.0 | 58.0 | 771-790 bp | 1.0 | 81.3 |
| trnM(CAU)-trnL(UAA)_p2 | TGAAGGAAAAGCCGGCTATC | TAGCGTCTACCAATTTCGCC | 50.0 | 50.0 | 58.0 | 58.1 | 779-798 bp | 1.0 | 80.8 |
| trnM(CAU)-trnL(UAA)_p3 | GCGATGCTCTAACCTCTGAG | ACCAATTTCGCCATATCCCC | 55.0 | 50.0 | 57.9 | 58.0 | 719-738 bp | 1.0 | 80.7 |
| trnM(CAU)-trnL(UAA)_p4 | GCGATGCTCTAACCTCTGAG | TAGCGTCTACCAATTTCGCC | 55.0 | 50.0 | 57.9 | 58.1 | 727-746 bp | 1.0 | 80.1 |
| trnM(CAU)-trnL(UAA)_p5 | GGAATCGAACCGATGACCAT | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 57.8 | 58.0 | 751-770 bp | 1.0 | 79.7 |